The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. Figure Legend Snippet: Changes in corticosterone baseline fecal cortisol/corticosterone metabolites (FCMs) from laboratory-bred deermice across the dark cycle measured with ( A ) corticosterone enzyme immunoassay (EIA) and ( B ) group-specific EIA. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticosterone(Cort). The microtiter plate provided in this kit has been pre-coated with Corticosterone(Cort) protein. Please read this insert completely prior to using the product. Switch to to see content relevant to your location. Sample Types Validated: Dried Fecal Extracts, Saliva, Urine, Extracted Serum/Plasma, and Tissue Culture Media. Arbor Assays Detectx Corticosterone Enzyme Immunoassay Kit, Arbor Assays() You are visiting ZAGENO United Kingdom, but you appear to be in United States. For measuring BECs, tail bleeding was performed once a week (Mondays) at 0700 h, the time of day corresponding to the last hour of ethanol exposure of CIE rats (Gilpin et al., 2008b). 1 Plate Kit Catalog Number K063-H1 5 Plate Kit Catalog Number K063-H5. Abstinence from prolonged ethanol exposure affects plasma corticosterone, glucocorticoid receptor signaling and stress-related behaviors. This assay employs the competitive inhibition enzyme immunoassay technique. DetectX Epiandrosterone Enzyme Immunoassay Kit. Calibrators, controls and diluted samples are incubated with enzyme (horseradish peroxidase) labelled corticosterone in the antibody coated wells overnight at 2-8C. The most sensitive, specific and flexible alternative to more costly and time-consuming methods for oxytocin detection.Įasy-to-use, well-validated serotonin ELISA kit for hormone research.Serum, plasma, urine and other biological fluids The Corticosterone EIA kit is a competitive enzyme immunoassay utilising a polyclonal corticosterone antibody coated onto the inner surface of polystyrene microtitre wells. and assayed using DetectX Corticosterone EIA kit (K014 Arbor Assays. Widely used sensitive cortisol ELISA kit with a large variety of sample types and highly reproducible results for stress, autoimmune disease and cancer research. In contrast, corticosterone induced a robust suppression of pulsatile LH secretion. DETECTX CORTICOSTERONE ENZYME IMMUNOASSAY KIT PDFHighly sensitive corticosterone ELISA kit, species independent, used for animal stress research.ĮLISA, Colorimetric detection | Print as PDF This product is compatible with the Absorbance 96 Plate Reader. Cited sample types include cell lysate, urine and whole blood.Ĭorticosterone (100%), Deoxycorticosterone (21.3%), Desoxycorticosterone (21.0%), Progesterone (0.46%), Testosterone (0.31%), Tetrahydrocorticosterone (0.28%), Aldosterone (0.18%), Cortisol (0.046%) and 5 minutes before diluting with EIA buffer.Īdd 380µl EIA assay buffer to each plasma tube, vortex.įinal dilution is ~1:40 (This final dilution may not be appropriate for every sample thus, optimal dilution must be determined by each end user for their experimental sample group.) determined using DetectX Corticosterone Enzyme Immunoassay Kit (Cat. For the quantitative determination of Corticosterone in culture supernatants, feces, plasma, serum, and saliva from any species. Corticosterone Immunoassay Kit Blood was collected from the inferior vena cava.
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